Document Details

Document Type : Article In Journal 
Document Title :
Evaluation of the Cytogenetic Status and DNA Integrity of Human Lymphocytes after Exposure to an Aqueous Extract of Rhazya Stricta Leaves in Vitro
Evaluation of the Cytogenetic Status and DNA Integrity of Human Lymphocytes after Exposure to an Aqueous Extract of Rhazya Stricta Leaves in Vitro
 
Subject : Biological 
Document Language : English 
Abstract : Human lymphocyte culture cells were treated with three different concentrations (6, 12 and 24 g/liter) of R. stricta aqueous leaf extract. Samples were collected at three different intervals (24, 48 and 72h) for each concentration. Following each treatment, cell samples were subjected to cytogenetic as well as comet test assays. The cytogenetic assay revealed a significant decrease in mitotic index that was inversely proportional with concentration and exposure time. Many aberrations, including high percentage of interphase, increased micronuclei, viscosity, colchicines metaphase and two nuclei-cells were detected. Microscopic observation showed necrosis of most of the treated cells at all used concentrations, an indicative possibility of anticancer activity. Comet test detected DNA lesions that were proportionally concentration- and exposure time-dependent too. The present data strongly suggest that the aqueous extract of the R. stricta leaves has mutagenic, clastogenic and possibly anticancer activities on human lymphocytes in vitro. 
ISSN : 1816-157X 
Journal Name : Journal of Applied Sciences Research 
Volume : 5 
Issue Number : 8 
Publishing Year : 2009 AH
2009 AD 
Article Type : Article 
Added Date : Sunday, March 21, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
نبيه عبد الرحمن باعشنBaeshen, Nabih AbdulrahmanResearcherDoctorate 
جمال صابر محمد صابرSabir, Jamal S.MResearcherDoctorateJsabir@Kau.edu.sa
صلاح الدين محمد ابو عباAbo-Aba, Salah al-Din MohammedResearcherDoctorate 
سمير قاريQari, Sameer Researcher  

Files

File NameTypeDescription
 26117.pdf pdfAbstract

Back To Researches Page